Oral Presentation 14th Australian Peptide Conference 2022

Three novel peptide families are the principal pain-causing constituents of stinging nettle venom (#45)

Irina Vetter 1 2 , Edward K Gilding 1 , Sina Jami 1 , Jennifer R Deuis 1 , Mathilde R Israel 1 3 , Peta J Harvey 1 , Aaron G Poth 1 , Fabian B.H Rehm 1 , Jennifer L Stow 1 , Samuel D Robinson 1 , Kuok Yap 1 , Darren L Brown 1 , Brett R Hamiliton 4 , David Andersson 3 , David J Craik 1 , Thomas Durek 1
  1. Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
  2. School of Pharmacy, The University of Queensland, Brisbane, QLD, Australia
  3. Wolfson Centre for Age-Related Diseases, Institute of Psychiatry, Psychology & Neuroscience, King’s College London, London, United Kingdom
  4. Centre for Advanced Imaging, The University of Queensland,, Brisbane, QLD, Australia

Stinging nettles, including the European common nettle Urtica dioca, the New Zealand Ongaonga stinging nettle Urtica ferox, and the Australian giant stinging tree Dendrocnide moroides, are renowned for causing pain following contact with the fluid-containing stinging hairs. Small molecule neurotransmitters, including histamine, serotonin, and acetylcholine, have been thought to mediate these painful sensations. However, injection of these compounds alone cannot recapitulate the severity and symptomatology of nettle stings, and stinging hair fluid devoid of these components still causes pain on injection. We recently discovered that peptides targeting neuronal membranes and ion channels mediate pain following nettle stings. These peptides are disulfide-rich and, in the case of compounds derived from D. moroides, resemble neurotoxins from spider and cone snail venom structurally. Our results provide an intriguing example of inter-kingdom convergent evolution of animal and plant venoms with shared modes of delivery, molecular structure and pharmacology.